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Adult mind N-CAMs have obvious molecular weights of 180,000 and 140,000 with an extra
type at 120,000 in murine brain. In distinction to the N-CAM found within the mind, however, only one polypeptide of Mr 140,000 wwas seen. In distinction to the two primary CAMs, Ng-CAM was found only on nerves supplying the feather and the skin.
Both trembler and med/med Schwaann cells stained intensely for cytotactin,
in distinction to normal Schwann cels which stained solely
slightly. During thhe formation of the feather filament,
N-CAM appeared periodically and asymmetrically on basilar cells situated in the valleys between adjacent barb
ridges. The potential relationship of cell adhesion to embryonic
induction during feathesr formation was eamined by immunohistochemical analysis oof the spatiotemporal distribution of three cell-adhesion molecules (CAMs), neural CAM (N-CAM),
liver CAM (L-CAM), and neuron-glia CAM (Ng-CAM), and of substrate molecules (laminin and fibronectin) in embryonic rooster
pores aand skin. Immunocytochemical methods were used to point
out thyat Ng-CAM (the neuron-glia cell adhesion molecule), N-CAM (the neural cell adhesion molecule), and the extracellular matrix protein cytotactin are extremely concentrated at nodes of
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